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KMID : 0613820030130050732
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Journal of Life Science
2003 Volume.13 No. 5 p.732 ~ p.739
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Effect of Co-Culture with Mammalian Spermatozoa on Maturation in vitro of Porcine Cumulus-Free Germinal Vesicle Oocytes
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Kim Byung-Ki
Kang Sung-Ryoung
Yim Cha-Ok
Han Chang-Hee
Lee Bok-Kyu
Lee Kwang-Sun
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Abstract
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The purpose of this study was to determine if the addition of spermatozoa into the culture medium could influence the nuclear maturation of denuded porcine germinal vesicle (GV) oocytes in vitro. Cumulus-oocyte complexes were collected from follicles of 3 to 5 mm in diameter. The cumulus and corona cells were removed from oocytes. Porcine denuded oocytes were cultured in tissue culture medium containing spermatozoa. After 48 h culture, oocytes were examined for the evidence of GV breakdown, metaphase I, anaphase-telophase I, and metaphase II (M II). The proportion of oocytes reaching M II stage was significantly (P＜0.01) increased in the oocytes cultured in media containing spermatozoa compared to those in media without spermatozoa (31.9±1.8% vs 14.9±1.0%). No differences in the rates of M II were observed among the different period of spermatozoa exposure nor among the spermatozoa from different species. The proportion of oocytes reaching M II stage was significantly different between high and low concentrations of spermatozoa. The present study suggests that mammalian spermatozoa contain a substance(s) that improves nuclear maturation in vitro of GV oocytes. Enhancing effect of spermatozoa for oocytes maturation in vitro is a highly dose-dependent.
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KEYWORD
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in vitro maturation, oocytes, porcine, spermatozoa
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